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KMID : 0379120120400030141
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Korean Journal of Mycology
2012 Volume.40 No. 3 p.141 ~ p.144
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Isolation and Identification of Yeasts from Wild Flowers of Daejeon City and Chungcheongnam-do in Korea
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Min Jin-Hong
Hyun Se-Hee
Kang Min-Gu
Lee Hyang-Bum
Kim Chang-Mu
Kim Ha-Kun
Lee Jong-Soo
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Abstract
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Total 50 species, 80 strains: Eighty strains of yeasts were isolated from wild flowers of Daejeon city and its surroundings in Korea. Isolated yeasts were identified by comparison of their PCR-amplified 18s rDNA V3 region or internal transcribed spacer-2 (ITS-2) genes with BLAST database. Among them, 32 species, 54 strains of yeasts were isolated and identified from wild flowers of Daejeon city, and Cryptococcus species were dominant yeasts. Furthermore, 18 species, 26 strains of yeasts were isolated and identified from wild flowers of Geumsangun and Nonsan-gun, Chungcheongnam-do in Korea, and also Candida species and Pseudozyma species were dominant yeasts.
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KEYWORD
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18s rDNA, BLAST database, ITS-2, PCR-amplify, Wild flowers, Yeast
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